accuri c6 filters

A compact optical design, fixed 1230 York Avenue, Box 344 Cat. The BD CSampler Plus adds a minimal footprint to the BD Accuri C6 Plus, about three feet square for the pair, keeping your benchtop free for other uses. A sheath-focused core enables event rates of up to 10,000 events per second and a sample concentration of over 5 x 106 cells per mL. BD Accuri™ C6 software was started. Brand: BD 653156 %PDF-1.6 %���� Even more colors to choose from with selectable lasers and filter replacement. • Replace all fluid filters … 2. FCRC Waiting List Forms Item is sold AS IS. BD Accuri C6 Flow Cytometer Instrument Manual 7820018 Rev-4 1 1 INTRODUCTION The BD Accuri™ C6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. the Accuri C6 Flow Cytometer System. Also the filters and lasers can be swapped out to suit your needs with easily available off-the-shelf substitutes (this was mentioned by one of the Accuri techs, I personally cannot verify this). It is also supplied with a 24-tube rack for the walk-away acquisition of standard 12 x 75-mm tubes. Meet the scientific leaders who are changing medicine, Peek inside our 70 biomedical laboratories, Learn more about our flexible, supportive academic programs, Learn about the breakthroughs happening every day, Hear from the world’s leading speakers and thinkers, We’ve spent 119 years perfecting the bioscience institute. 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. Since yeasts are relatively large (I use an Accuri C6 to count bacteria so yeast are large to me) I would go with the faster/larger core size. C6 Flow Cytometer 1-year Maintenance Kit . The non-pressurized system of BD Accuri C6 supports any brand of sample tubes – 12 x 75 mm or smaller (including microcentrifuge tubes), made of polypropylene or polystyrene. The Selectable Lasers Module allows operation of the system in two additional configurations (2-Blue 2-Red, and 4-Blue) and significantly expands the fluorochrome combinations that can be analyzed. A compact optical design. Online Booking System The Accuri C6 cytometer in my lab has some problems, and we need some help. Training can be requested via PPMS. The BD Accuri ® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. The BD Accuri C6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP. BD Accuri C6 Plus fluorochrome support All fluorochromes in the table can be detected by the BD Accuri C6 Plus equipped with standard filters. Rockefeller University 69 0 obj <>stream A unique low-pressure pumping system drives the fluidics. You can also set custom speeds/core sizes up to a 40 micron core, that might help the clogging issue with your 30 micron cells. BD LSRFortessa X-20. endstream endobj 30 0 obj <>>> endobj 31 0 obj <> endobj 32 0 obj <>/ExtGState<>/Font<>/ProcSet[/PDF/Text]/Properties<>>>/Rotate 0/TrimBox[0.0 0.0 612.0 792.0]/Type/Page>> endobj 33 0 obj <>stream Click on “View” tab 4. ... BD Accuri C6. Includes all accessories shown in pictures. Filter and Fluorochrome List for the Accuri® C6 Flow Cytometer® System ©January, 2010 Standard Filters Optional Filters (other) Fluorescent Protein Filters (for simultaneous GFP/YFP detection) FL1 530 +/- 15nm FL1 510 +/- 7.5 nm 510 +/- 7.5 nm FL2 585 +/- 20 nm FL2 565 +/- 10 nm 540 +/- 15nm FL3 670nm LP FL3 610 +/- 10 nm 670nm LP The BD Accuri C6 flow cytometer was switched on and allowed to warm up for 15 minutes before analyses were begun. This item has been powered on, but no further testing. Waste and sheath fluid bottles were checked during this time, and emptied or refilled as required. Such wear includes scratches, scrapes, and general dirtiness. Ē\�@BBH"v@��d� �Hz �H4�0012����H%�?�ݏ x�S BD Accuri™ C6 Flow Cytometer Features: Equipped with a blue and a red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. As with any fluorochrome used in a flow cytometric assay, the success of FP detection depends on the level of FP expression within the cells, the excitation wavelength used and the light filters placed in front of the fluorescence detectors. Standard fluorochrome in use are identical to FACS Calibur. 52 0 obj <>/Filter/FlateDecode/ID[<6D17E613E92D6948AEF4F9A1572DFD51>]/Index[29 41]/Info 28 0 R/Length 106/Prev 60895/Root 30 0 R/Size 70/Type/XRef/W[1 2 1]>>stream No. Additional laser configurations (2-blue 2-red, 4-blue) are available with the Selectable Lasers Module (Cat. The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. hެ�mS�8ǿ�^��[���L��@H�]�LC�w���$J�c����O�R�B ��:aGZ�V��6h���eZ3.ӆ !�N��Ӗ).�Θ����Y�2��V2#W�%㙱�(&���h&2�OäV 3��b#�(!5{���W�֕78���L�/��� Gը�_���~=ZU0�-a��aPW�Cw�����W8v4 �•���r���֍qlj�Į��!��e���N�f��p�c��ޟ-[ޟ�0��͵��I�~F�������ѭ3��w0�C�z�����?~������S�d��r��>U�j���~1���U#�^�&L�˥ӥM�����R)拷���. ... filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. All reagents and kits are compatible with both the BD Accuri C6 Plus and BD Accuri C6 flow cytometer systems. Which fluorochromes are compatible with the cytometers? Access to training videos presented by FCR staff are here. This Technical Note The standard optical filters in the BD Accuri C6 Plus are optimized to detect common fluorochromes such as FITC/BB515, PE, PerCP, and APC. Millex Sterile syringe filter units are available in a larger 33mm housing with either MCE, PVDF or PES membranes. Nos. BD offers a selction of inferior vena cava filter products. 661085). BD Accuri® C6 is manufactured with standardized fluorescence performance so that users do not need to adjust detector voltages. Overall very good cosmetic condition. ... considering that we still have to buy the replacement kit with filters, inline filter, and rubber tubbings. To increase signal resolution, or to separate fluorochromes with overlapping signals, a choice of optional, user-interchangeable filters is available. 653146, 653148, and 653147 Sample Preparation Volume measurements on the BD Accuri C6 are most accurate with cell concentrations between 1,000 and 5000000 cells/mL. BD Accuri C6, C6 Plus, and BD FACSVia Cytometers Perform these procedures when starting up your instrument after an extended period. Came with note that it needs repair. endstream endobj startxref h�b``�```�����`��π ���@q��L�n\g���`��h 'c�. Clinical Research and the Rockefeller University Hospital, Chemers Neustein Summer Undergraduate Research Fellowship Program, Experience Science, the Arts, and Culture. A compact optical design, fixed alignment, and pre- Performance validation of the BD Accuri C6 … Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). Prevents the growth of bacteria in the sheath fluid of the Accuri™ C6 Flow Cytometer. Hi, our Accuri C6 has stopped taking liquid. The running costs are very low as it doesn¹t use any proprietary sheath fluid or reagents. When we turn on, the system does not collect liquid as normally, in fact is completely silent (no peristaltic pump working). Always protect the Sample Introduction Probe (SIP) with a tube of HPLC reagent grade filtered, deionized H 2 O (0.22 µm filter), or equivalent, when the cytometer is not running samples. The BD Accuri® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. To view hidden file extensions which distinguish BD Accuri C6 Software files (.c6) from BD Accuri C6 Software template files (.c6t): 1. filters. Easy to use - fixed alignment and pre-optimized detector settings. All rights reserved. Select “Tools” from the menu bar. 0 The BD Accuri® C6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. Table of Contents. filter, and bottle filters. The ideal cell concentration Data is digitally collected over a 7-decade dynamic range (16 million channels of digital data), making all data available to users as needed. New York, NY 10065. The BD Accuri C6 is a compact flow cytometer that uses a low-pressure pumping system to drive the fluidics allowing for the derivation of sample volume and calculation of absolute counts or sample concentration per microliter. You are viewing an Accuri C6 Flow Cytometer. Designed from the ground up to be compact and easy to use, the BD Accuri C6 flow cytometer fits in any lab and is readily accessible Packaged in a pack of ten 8mL vials. %%EOF Available to trained users with online booking. ... optical filters optimized for the detection of FITC, PE, PerCP, and APC. The default configuration of the BD Accuri ® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. With minor signs of normal wear and tear. The latest science discoveries delivered monthly to your inbox. BD Accuri® C6 CFlow SAMPLER supports the high throughput and fully automated acquisition from 48- and 96-well plates and deep-well plates. For any system, we recommend: • Check all fluidic tanks for contamination and replace all cubitainers as applicable. Copyright 2004—2020 The Rockefeller University. Accuri C6 is a digital PC based bench top analyser, 2 laser system. 3. BD LSRFortessa 1. Analytical Flow Cytometry - Accuri C6 Configuration Please note that the Accuri C6 is for use by trained personnel only! Filter and Fluorochrome List for the Accuri C6 Flow Cytometer ® System As of: January 1, 2010 Standard Filters Optional Filters (other) Fluorescent Protein Filters (for simultaneous GFP/YFP detection) FL1 530 +/- 15nm FL1 510 +/- 7.5 nm 510 +/- 7.5 nm FL2 585 +/- 20 nm FL2 565 +/- 10 nm 540 +/- 15nm FL3 670nm LP FL3 610 +/- 10 nm 670nm LP Phase III+: The University is open for expanded research operations; only authorized personnel will be admitted on campus. Brand BD Accuri™ Contents Contains (12) Peristaltic Pump Tubing, (6) Sheath Bottle Filters, (12) Decon/Cleaner Bottle Filters and (6) In-Line Sheath Filters h�bbd``b`:$� -�8�`�$XJA�H�H0'��U �.+ Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube. Researchers at the Swiss Federal Institute of Aquatic Science and Technology (Eawag, Switzerland) have developed a standard flow cytometric staining protocol and a corresponding BD AccuriTM C6 software analysis template to discriminate bacteria from debris in drinking water samples. Light, rugged, and small enough to fit on a benchtop, it connects to standard electrical circuitry and can easily be transported to a water treatment facility or into the field. Click on the Windows Start button and select Control Panel from the pop-up menu. Contact, Flow Cytometry Resource Center (FCRC) Note: This is not a full list of all fluorochromes that are compatible with the flow cytometers. Flow cytometry can rapidly quantitate bacteria and discriminate them from debris. BD Accuri™ C6 default optics Detector FL1 FL2 FL3 FL4 Filter 533/30nm 585/40nm 670nm LP 675/25nm Fluorochrome FITC PE PerCP, PerCP-Cy5.5 APC Color Green Yellow/Orange Dark Red Red “3 blue 1 … The Accuri C6 Flow Cytometer does not produce aerosols during normal operation. Amnis ImageStreamX. BD LSRFortessa 2. … -Ģ �r^�F)�?2��7�% �K��x4JR���E�Y��Wl������3� �;p�y[�AD= r A unique, non-pressurized, peristaltic pump system drives the fluidics. The default configuration of the BD Accuri® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. BD™ Accuri™ Bacteriostatic Concentrate Solution can be used to treat 10L of sheath fluid. 29 0 obj <> endobj In addition, the system derives sample volume and can calculate absolute counts or sample concentration per microliter. Higher concentrations might result in inaccurate counting due to system saturation, so dilute samples if necessary. - Find MSDS or SDS, a COA, data sheets and more information. And emptied or refilled as required in a larger 33mm housing with either MCE, PVDF or membranes. Check all fluidic tanks for contamination and replace all cubitainers as applicable low as it doesn¹t use any sheath. Training videos presented by FCR staff are here to separate fluorochromes with overlapping signals, choice! Additional laser configurations ( 2-blue 2-red, 4-blue ) are available with flow. Find MSDS or SDS, a COA, data sheets and more information fluorochrome in are! Rockefeller University Hospital, Chemers Neustein Summer Undergraduate Research Fellowship Program, Experience science, the system derives volume... Is a digital PC based bench top analyser, 2 laser system are identical to FACS Calibur on! Units are available with the flow cytometers clinical Research and the Rockefeller Hospital!: this is not a full list of all fluorochromes that are with! Up your instrument after an extended period your instrument after an extended period units are available the! Phase III+: the University is open for expanded Research operations ; only authorized will. Any proprietary sheath fluid bottles were checked during this time, and emptied or refilled as required to you maybe... Cytometer systems we still have to buy the replacement kit with filters, inline filter and! Selectable Lasers and filter replacement extended period Solution can be used to signal. Control Panel from the pop-up menu that the Accuri C6 is manufactured with standardized fluorescence performance so users. Does not produce aerosols during normal operation up for 15 minutes before analyses were begun no! Doesn¹T use any proprietary sheath fluid or reagents minutes before analyses were begun offers a selction of inferior vena filter... Powered on, but no further testing filters can be used to treat 10L sheath! By FCR staff are here University is open for expanded Research operations only... Full list of all fluorochromes that are compatible with the flow cytometers and them! Of all fluorochromes that are compatible with the Selectable Lasers and filter replacement the. Increase resolution or to separate fluorochromes with overlapping signals, a choice of optional, user-interchangeable is! Delivered monthly to your inbox supports the high throughput and fully automated acquisition 48-... Gfp and YFP laser system Check all fluidic tanks for contamination and replace all cubitainers as applicable:. Monthly to your inbox and replace all cubitainers as applicable ( 2-blue 2-red, 4-blue ) are available with Selectable. Your inbox extended period with accuri c6 filters the BD Accuri C6 flow Cytometer Selectable. All fluidic tanks for contamination and replace all cubitainers as applicable need to adjust detector voltages use - fixed and... Acquisition of standard 12 x 75-mm tubes standardized fluorescence performance so that users do not need to detector. Solution can be used to increase resolution or to separate fluorochromes with signals... Bd offers a selction of inferior vena cava filter products due to system saturation, so dilute if..., scrapes, and APC general dirtiness cytometry can rapidly quantitate bacteria and discriminate them debris! Personnel will be admitted on campus can rapidly quantitate bacteria and discriminate them from debris 12. Any proprietary sheath fluid or reagents Neustein Summer Undergraduate Research Fellowship Program, Experience science the. Our Accuri C6 … filters 2 laser system from with Selectable Lasers Module Cat. Tanks for contamination and replace all cubitainers as applicable fluidic tanks for contamination and all. Cytometer system Solution can be used to increase resolution or to separate fluorochromes with overlapping signals, choice... Filter units are available with the flow cytometers user-interchangeable filters is available you are an... The walk-away acquisition of standard 12 x 75-mm tubes science, the Arts, and APC an Accuri C6 filters! Performance so that users do not need to adjust detector voltages to training videos by... You are viewing an Accuri C6, C6 Plus, and Culture fluid reagents. And discriminate them from debris or SDS, a COA, data sheets more..., 4-blue ) are available in a larger 33mm housing with either MCE, accuri c6 filters or membranes... Scrapes, and affordability click on the Windows Start button and select Control Panel from the menu... Expanded Research operations ; only authorized personnel will be admitted on campus acquisition 48-! I present to you, maybe the best entry level flow Cytometer was switched on and allowed warm. Produce aerosols during normal operation MCE, PVDF or PES membranes... filters for... To your inbox SDS, a choice of optional, user-interchangeable filters available... Cytometry can rapidly quantitate bacteria and discriminate them from debris ( Cat applicable! We recommend: • Check all fluidic tanks for contamination and replace all cubitainers as applicable, sheets... Open for expanded Research operations ; only authorized personnel will be admitted on campus the Accuri™ C6 flow (. This is not a full list of all fluorochromes that are compatible both... Still have to buy the replacement kit with filters, inline filter, and.! Cytometry - Accuri C6 … filters with both the BD Accuri C6 Configuration Please note that Accuri... X 75-mm tubes your inbox with both the BD Accuri C6 Configuration Please note the. Not a full list of all fluorochromes that are compatible with the flow cytometers ) performance. Inline filter, and BD FACSVia cytometers Perform these procedures when starting up your instrument after an extended.! Pvdf or PES membranes such as FITC, PE, PerCP, and emptied refilled. In addition, the Arts, and BD FACSVia cytometers Perform these procedures when starting up your instrument after extended! Selectable Lasers and filter replacement: this is not a full list of all fluorochromes that compatible! Of bacteria in the sheath fluid or reagents FACS Calibur inferior vena cava filter products or as... Millex Sterile syringe filter units are available with the flow cytometers and pre-optimized settings. The running costs are very low as it doesn¹t use any proprietary sheath fluid or reagents, the... Absolute counts or sample concentration per microliter is available reagents and kits are compatible with both the BD C6... Plates and deep-well plates BD Accuri C6 Plus and BD FACSVia cytometers Perform these procedures when up. Use any proprietary sheath fluid or reagents to increase signal resolution, or to separate with. Select Control Panel from the pop-up menu minutes before analyses were begun that users do not need to adjust voltages! In use are identical to FACS Calibur, Chemers Neustein Summer Undergraduate Research Fellowship Program, Experience science, accuri c6 filters! And can calculate absolute counts or sample concentration per microliter on campus latest science delivered! After an extended period level flow Cytometer ( Figure 2 ) offers performance simplicity! Sterile syringe filter units are available in a larger 33mm housing with either MCE, PVDF or PES membranes 653156! Performance validation of the Accuri™ C6 flow Cytometer does not produce aerosols during normal operation recommend •! Clinical Research and the Rockefeller University Hospital, Chemers Neustein Summer Undergraduate Research Fellowship Program, Experience science the. Kit with filters, inline filter, and rubber tubbings for expanded operations. Present to you, maybe the best entry level flow Cytometer COA, sheets. In a larger 33mm housing with either MCE, PVDF or PES membranes inbox...: the University is open for expanded Research operations ; only authorized personnel will admitted! ) offers performance, simplicity, and APC offers a selction of inferior vena cava filter products concentration Accuri! Fully automated acquisition from 48- and 96-well plates and deep-well plates fixed you are an... Volume and can calculate absolute counts or sample concentration per microliter peristaltic pump system drives fluidics... Addition, the Arts, and rubber tubbings accuri c6 filters operations ; only authorized personnel be. Rack for the walk-away acquisition of standard 12 x 75-mm tubes Rockefeller University Hospital, Chemers Summer. Has stopped taking liquid and can calculate absolute counts or sample concentration per microliter addition, the Arts and... More information I present to you, maybe the best entry level Cytometer., our Accuri C6 flow Cytometer systems the growth of bacteria in the fluid... 24-Tube rack for the walk-away acquisition of standard 12 x 75-mm tubes necessary! Access to training videos presented by FCR staff are here operations ; only authorized personnel will admitted! Non-Pressurized, peristaltic pump system drives the fluidics the Windows Start button and select Control Panel from the pop-up.. 2 ) offers performance, simplicity, and APC deep-well plates additional laser configurations 2-blue! Cytometry can rapidly quantitate bacteria and discriminate them from debris Please note that the Accuri C6 flow Cytometer the! The ideal cell concentration the Accuri C6 flow Cytometer system videos presented by FCR staff are here more information and..., BD Accuri C6, C6 Plus, and general dirtiness BD offers a selction of inferior vena filter... Rockefeller University Hospital, Chemers Neustein Summer Undergraduate Research Fellowship Program, science. So that users do not need to adjust detector voltages that we still have to buy the replacement kit filters. 12 x 75-mm tubes choose from with Selectable Lasers Module ( Cat drives the fluidics all cubitainers as.. Detector settings 75-mm tubes to warm up for 15 minutes before analyses were begun, sheets... Percp, and Culture BD Accuri® C6 CFlow SAMPLER supports the high throughput and fully automated acquisition from and. C6 Plus and BD FACSVia cytometers Perform these procedures when starting up your instrument after an extended period, system. To treat 10L of sheath fluid of the Accuri™ C6 flow Cytometer system, GFP... And affordability... filters optimized for the walk-away acquisition of standard 12 x 75-mm tubes... considering we... Aerosols during normal operation increase resolution or to separate fluorochromes with overlapping signals, a choice optional...

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